Animal cell tech
DEPARTMENT OF BIOCHEMISTRY & MOLECULAR BIOLOGY
BMED 447: ANIMAL CELL CULTURE AND BIOTECHNOLOGY
1.(a) Animal tissue & cell culture
(c) Interrelationships between (a) and (b) and significance (IL)
2.Cell lines in Biomedical Research
(d) Primary cell lines,their advantages and disadvantages
(e) Production process.
3.Mammalian cell culture and production process
(a) Application of Biotechnology in production of mammalian cell cultures
(i) Primary cells as raw materials
(ii)Condition for cell culture:aseptic media,aeration,freezing and storage etc.
(iii) Primary culture,cell lines and cloning
(iv) Isolation of issues
(v) Enzymatic degradation
(vi) Mechanical disaggregation
(vii) Slide coverslip culture
(viii) Flask culture (6 L)
- Separation of viable and non viable cells and cell counting (IL)
- Laminer-flow cabinet & modification (IL)
- Metabolism of animal tissue culture (2 L)
7.Characteristic and growth of animal cell cultures
(b) Continuous culture
(c) Fed-back culture
8.Bioreactiors for animal’s cells, monitoring and control of bioreactors in animals cell cultures.
(a) Importance of bioreactors for animal cell culture
(b) Chemostats and turbiostats
(c) Gas-distribution bioreactors
(d) Stirred DNA technology of animal cell
9.Recombinant DNA technology of animal cell
(a) Overview of recombinant DNA and recombinant DNA technology
(i) Recombinant DNA and significance
(ii) Vectors,restriction enzymes,and clones
(iii) Gene splicing and synthesis of new proteins
(iv) Hybridoma; production & selection(4 L)
10.Genetic engineering of Animals cells
(b) Bulk production of proteins by genetically engineered animal cells
(c) Genetically engineered proteins in medicine, agriculture and industries(3 L)
(a) Definition and significance
(b) Processing for developing transgenic animals.
12.Down stream processing and protein production recovery
(a) Definition and significance
(b) Processing steps
13.Blood serum fractionation and growth factors
(a) Overview of blood plasma, serum etc.
(b) Functions and origin of plasma proteins
(c) Fractionation of serum proteins
(d) Growth factors
(i) Definition and characteristic features
(ii) Nerve growth factor (NGF)
(iii) Epidermal growth factor (EGF)
(iv) Transgenic growth factory (TGF) BI,B2, B2
(v) Platelet-derived growth factors (PDGF)
(vi) Fibroblasts growth factors(PDGF)
(vii) Others peptides(9L)
FIRST SEMESTER 2014/2015 ACADEMIC YEAR.
-Attempt all questions in section A and any other TWO questions in section B.
-Use pencil where applicable.
- i) Outline the functions and structure of the following organelles (4mks)
a) Rough endoplasmic reticulum
b) Golgi apparatus
ii) explain the functions of any two plastids found in plant cell (2mks)
- a) Identify three major classes, composition of filaments that make up cytoskeleton and state the functions of each. (6mks)
b) Explain why the three classes of filaments that make up cytoskeleton would be plentiful in a muscle cell. (4mks)
- Mitosis is divided into four stages;
a) In which of the stages of mitosis does spindle fibres contract into visible structures.
b) outline the functions or mitosis
c) How do the first and second meiotic divisions differ.
d) Outline possible errors and consequences of meiotic divisions. (4mks)
4.Discuss the structure and functions of glycocalyx and how it is important to both prokaryotic and eukaryotic cell (8mks)
5.a)Outline the causes of cellular aging and cell death (4mks)
b) Differentiate between chloroplasts and mitochondria (4mks)
6.Discuss the structure of mitochondria and process of energy production (15mks)
7.Discuss various systems of junctional complexes in animal cell: their structure and function. (15mks)
8.Discuss various ways by which substances enter and leave a cell. (15mks)
KABARAK UNIVERSITY EXAMINATIONS
2008/2009 ACADEMIC YEAR
FOR THE DEGREE OF BACHELOR OF ENVIRONMENTAL SCIENCE
COURSE CODE: BOTA 415
COURSE TITLE: CELL BIOLOGY
STREAM: SESSION V & VII
Answer All questions in Section A and Any TWO from Section B
SECTION A. (40 MARKS)
- (a) State four differences between plant and animal cells. (5 Marks)
(b) State one function of each of the following cell organelles. (4 Marks)
(i) Endoplasmic reticulum
(ii) Golgi apparatus
- Distinguish between Prokaryotic and Eukaryotic cells. (8 Marks)
- (a) Differentiate between pinocytosis and Phagocytosis. (2 Marks)
(b) State the differences between diffusion and active transport. (6 Marks)
- (a) Explain giving examples what plastoglobuli are (4 Marks)
(b) State the four components of Plastids (4 Marks)
- (a) Compare and contrast features of mitochondria and chloroplast. (6 Marks)
(b) Name two functions of Thylakoids. (2 Marks)
SECTION B. (30 MARKS)
- Describe the Describe the composition of plasma membrane as proposed by Singer and
Nicolson. (15 mks)
- Describe the chemical composition and functions of higher Eukaryotic cell wall.
- Give an account of various types of specialized cells using appropriate examples.