UNIVERSITY EXAMINATIONS 2007/2008
INSTITUTE OF OPEN LEARNING
SPECIAL/SUPPLEMENTARY EXAMINATION FOR THE DEGREES OF
BACHELOR OF EDUCATION AND BACHELOR OF SCIENCE
SBT 202: GENERAL MICROBIOLOGY
DATE: Friday 10th October, 2008
Answer ALL questions in Section A and any ONE question in Section B. Answers to
questions in Section A should be concise, and should be written in the spaces provided in
the question papers.
SECTION A (5 marks each)
1.Describe the different morphological forms of viruses.
2.State the contribution of the following individuals to microbiology.
(a) Hans Christian Gram
(b) Ernest Abbe
(c) Robert Hooke
(d) Roger Stanier
(e) Von Behring
3.State five uses of alginates.
4.Describe the different types of pili in bacteria.
5.Describe the structure of gram negative cell in wall.
6.State the importance of the following constituents of culture media
7.State the functions of the following structures in bacteria
(b)Capsules and slime layers.
8.Outline the techniques of heat fixation of bacterial smears.
SECTION B: ESSAY QUESTIONS (30 MARKS)
9.Discuss the use of moist heat in control of microorganisms.
10.Describe the life cycle of Rhizopus stolonifer.
11.Discuss the economic importance of fungi and bacteria to man.
MAIN END SEMESTER EXAMS
Attempt all questions in section A,and any two question from section B
SECTION A 40 MARKS
1.Define the following terminologies as applied in the field of microbiology:(5 marks)
2.Explain on the contribution of Francesco Redi in disapproval of spontaneous generation. (5marks)
3.List and explain on the various means of sexual reproduction in fungi. (5marks)
4.Write notes on salient features of the protozoan. (5marks)
5.Account on the general morphology of viruses. (5marks)
6.Explain the various types of culture media and their importance. (5marks)
7.What is an antibiotic? Discuss how microorganisms can develop resistance to an antibiotic. (5marks)
SECTION B:30 MARKS
8.(a)Write an account on the microscopic methods used for stained and unstained preparation commonly used in the study of microorganisms. (10marks)
(b)Give an account on acid fast staining. (5marks)
9.(a)Discuss on the various conditions influencing the efficacy of an antimicrobial agent. (10 Marks)
(b)Enumerate the modes of action of various disinfectants. (5 marks)
10.(a)Explain the five steps typical of the lyric cycle of viral replication. (10 marks)
(b)Briefly describe various ways in which viruses may be cultivated. (5 marks)
FIRST YEAR EXAMINATION FOR THE AWARD OF DEGREE OF
BACHELOR OF SCINCE (ANIMAL SCIENCE), BACHELOR OF SCIENCE (AGRICULTURE), BACHELOR OF SCIENCE (GENERAL) BACHELOR OF SCIENCE (ENVIRONMENTAL SCIENC, BACHELOR OF EDUCATION (SCIENCE), BACHELOR OF SCIENCE (HORTICULTURE) , BACHELOR OF SCIENCE(NARE), BACHELOR OF SCIENCE (WILDLIFE), BACHELOR OF SCIENCE (ECOTOURISM
BOTA 131: GENERAL MICROBIOLOGY
STREAMS: BSC (AN SC, AGRIC, HORT, NARE, WILD, GEN, ENV. SCI) Y1S2 TIME: 2 HOURS
DAY/DATE: WEDNESDAY 7/8/2013 2.30 PM 4.30 P
SECTION A – 30 MARKS (Answer all the Questions)
- State the role of each of the following structures of a bacterial cell.
- (a) Outline four groups of bacteria on the basis of temperature requirements.
(b) Give three examples of differential stains used in identification of bacteria.
- (a) Explain the different types of asexual spores produced by fungi. [5 marks]
(b) Explain the difference between Homokaryotic and a Heterokaryotic cell in fungi. [3 marks]
- (a) State and explain the techniques used in cultivation of viruses. [6 marks]
- Outline the salient features of the protozoan class ciliophora. [4 marks]
- Give the microbial and chemical contaminants of water. [4 marks]
SECTION B- 40 MARKS (Answer any Two Questions)
- Describe the biotechnology products obtained through microbial activities. [20 marks]
- Discuss the factors that contribute to the emergence and re-emergence of infectious diseases. [20 marks]
- Write an essay on the applications of Microbiology in modern day life. [20 marks]
UNIVERSITY EXAMINATIONS 2011/2012
SECOND SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR
OF SCIENCE AND BACHELOR OF EDUCATION
DATE: Friday, 30th March, 2012
TIME: 11.00 a.m. – 1.00 p.m.
Answer ALL questions in Section A and any ONE question in Section B. Answers to
question in Section A should be concise and should be written in the spaces provided in
the question paper.
SECTION A: (5 MARKS EACH)
- State ONE contribution of the following persons in the evolution of microbiology
a) John Needham.
b) Martinus Beijerinck.
c) Richard Petri.
d) Robert Koch.
e) Watson and Crick.
- Explain various ways in which infections may be acquired in the laboratory.
- a) Distinguish between Indicator and Transport media giving ONE example media in each case.
b) Describe the use of anaerobic jar in the laboratory
- a) Describe bacterial colony characteristics on agar plate.
b) Outline the technique of preparation of bacterial smear from agar
- a) Classify bacteria according to air requirements for growth.
b) Name three types of energy sources for microbes and give an example
microbe of each.
- State the characteristics of Mycoplasmas and Actinomycetes.
- Outline the following methods of bacteria enumeration
a) Spread plate.
b) Direct microscopic bacterial count.
- a) Describe the stages of bacterial spore formation
b) Draw labeled diagrams showing different flagella arrangements in bacteria.
SECTION B: ESSAY QUESTIONS (30 MARKS EACH).
Answer any ONE question in this Section.
- Discuss methods of reproduction in fungi.
- Write an essay on disinfectants giving examples and stating their uses.
- Discuss the control of microorganisms using dry heat and radiations.
UNIVERSITY OF KABIANGA
2014/2015 ACADEMIC YEAR
SECOND YEAR SECOND SEMESTER EXAMINATION
FOR THE DEGREE OF BACHELOR OF SCIENCE IN MICROBIOLOGY
COURSE CODE: MIC 210
COURSE TITLE: GENERAL MICROBIOLOGY
TIME: 2.00 P.M- 5.00 P.M
INSTRUCTIONS TO CANDIDATES:
Answer ALL Questions in section A and any other THREE in section B.
SECTION A (28 MARKS): ATTEMPT ALL QUESTIONS
- Most organisms are neutrophils. Explain. (2 marks)
- Give a brief description of various shapes of found in bacteria. (5 marks)
- Explain what aseptic technique means to a microbiologist. (2 marks)
- a. What is a colony? (2 marks)
b. Clearly provide advantages and disadvantages of streak method of inoculation. (4 marks)
- State the functions of bacteria cell wall. (3 marks)
- Using a table, explain the difference between prokaryotic and eukaryotic cells. (4 marks)
- Explain basic technique necessary in isolation and culturing of microorganisms. (6 marks)
SECTION B (42 MARKS): ANSWER ANY THREE QUESTIONS
- Illustrate the life cycle of lytic phage. (14 marks)
- a. Explain the basis for virus classification. (6 marks)
b. Draw and label a generic virus. (8 marks)
- Describe in detail the structure and functions of plasma (cytoplasmic) membrane In a
bacterium. (14 marks)
- Discuss the physical factors influencing bacterial cultivation and growth. (14 marks)
1) describe the DNA structure using the watson-crick model
2) describe the germ theory of disease
3) breifly describe the principle of staining
4) describe the membrane protein of prokaryotes
5) define taxonomy and explain the Linnean scheme
6) describe asexual reproduction in microbes
7) how can we preserve food
8) describe three typed of Germi ides
SECTION B(ESAY QUESTIONS, 30 marks)
9) describe microbial growth under the following headlines,phase of growth,techniques used to measure number of microbes and factors affecting growth of microorganism
10) discuss the concept of microscopy under the following headings
a)principle of microscopy
b)types of microscope
c)techniques of light microscopy
11) describe the physical control of microbial control
University Examinations 2013/2014
SECOND YEAR, SECOND SEMESTER EXAMINATION FOR THE DEGREE OF BACHELOR
OF SCIENCE IN HORTICULTURE
AHS 2102: GENERAL MICROBIOLOGY
DATE: DECEMBER 2013 TIME: 2HOURS
INSTRUCTIONS: Answer question one and any other two questions
QUESTION ONE – (30 MARKS)
a) Define the following terms as used in applied bacteriology. (6 Marks)
iv. Thermal death point
v. Thermal death time
vi. Taq Polymerase
b) Outline the Koch postulates which help identify the etiological cause of disease and state cases
where they do not apply. (8 Marks)
c) Describe the major steps involved in replication of animal viruses. (8 Marks)
d) Giving an example in each case, describe vascular wilt fungi and the rust fungi.
QUESTION TWO (20 MARKS)
a) State four important uses of alginate. (2 Marks)
b) Describe the cell structure of gram positive bacteria. (3 Marks)
c) Using an example of your choice, describe an agricultural enterprise based on application of
microbiology. (5 Marks)
d) Describe streak plate method of isolating a pure bacteria culture from a mixture.
QUESTION THREE (20 MARKS)
a) Describe mycorrhiza fungi. (5 Marks)
b) Outline the most common ingredients of culture media. (5 Marks)
c) Discuss control of micro-organisms using dry and moist heat methods. (10 Marks)
QUESTION FOUR (20 MARKS)
a) Describe how resistance (R) genes work against plant viruses. (5 Marks)
b) Discuss economic importance of micro-organisms. (15 Marks)
QUESTION FIVE (20 MARKS)
a) Differentiate between the root – knot nematodes and cyst nematodes. (5 Marks)
b) Discuss the general considerations for sterilization and disinfection. (15 Marks)